NodoMap: A single-cell and spatial transcriptomic atlas of the mouse nodose ganglion.

The vagus nerve is a central component of the parasympathetic nervous system, innervating multiple abdominal organs to monitor and regulate their function. Vagal sensory neuron cell bodies reside in the nodose ganglia, but their molecular diversity and spatial organization remain incompletely defined. Here, we generated and integrated single-nucleus RNA sequencing (snRNA-seq) data with multiple published datasets to construct a unified atlas of 106,436 cells and combined this with spatial transcriptomics to create a single-cell and spatial map of the mouse nodose ganglion (NodoMap). We identify 21 neuronal subtypes and multiple non-neuronal populations, revealing subtype-specific gene expression differences between left and right ganglia and transcriptional responses to fasting. Spatial analysis shows intermingled neuronal populations with distinct cellular neighborhoods. Together, NodoMap provides a high-resolution resource for dissecting vagal sensory circuits and identifying molecular targets involved in energy homeostasis and metabolic disease.