Generating 3D mouse organoids for cortex development and evo-devo.

How do ci-regulatory differences, across mouse subspecies and over evolutionary timescales, shape cell-type specification and maturation during corticogenesis? In this issue of Developmental Cell, Medina-Cano et al. establish a scalable mouse organoid platform that recapitulates developmental dynamics and enables mapping of allele-specific expression, linking cis-regulatory variation with neurodevelopmental mechanisms and disease-associated genetics.