TIGR on the loose: A dual-guide RNA system for DNA targeting.
Molecular cell, 85(9): 1712-1713 PMID 40315825 DOI 10.1016/j.molcel.2025.04.007
Abstract
A recent study1 unveils tandem interspaced guide RNAs (TIGRs) that simultaneously engage both strands of target DNA and direct Tas protein activity. It offers insights into the evolution of RNA-guided proteins and introduces a promising tool for genome editing.