Driving the wedge: Understanding an improved Cas9 to better engineer others.
Molecular cell, 84(12): 2221-2222 PMID 38906114 DOI 10.1016/j.molcel.2024.05.026
Abstract
In their recent structural work, Eggers et al.1 rationalize how key mutations in the WED domain of the compact and thermostable Geobacillus stearothermophilus Cas9 bolster its editing efficiency in mammalian cells, and they use these insights to rationally improve another Cas9.