Broadening the targetable space: engineering and discovery of PAM-flexible Cas proteins.
Trends in microbiology, 32(8): 728-731 PMID 38816311 DOI 10.1016/j.tim.2024.05.004
Abstract
The application of CRISPR-Cas systems has been hindered by their requirement for long protospacer-adjacent motifs (PAMs). Recent engineering and discovery of PAM-flexible Cas proteins have substantially broadened the targetable DNA sequence space, thereby facilitating genome editing and improving derivative technologies such as gene regulation, seamless cloning, and large-scale genetic screens.