Streamlining mouse genome editing by integrating AAV repair template delivery and CRISPR-Cas electroporation.
Lab animal, 53(5): 115-116 PMID 38600182 DOI 10.1038/s41684-024-01363-w
Abstract
Recent genome editing techniques have substantially simplified the generation of genetically modified mice. A new study combines adeno-associated viruses (AAV) and electroporation to generate a robust pipeline to deliver CRISPR-Cas reagents into mouse embryos.