High-throughput creation of Nicotiana tabacum gene-targeted mutants based on CRISPR/Cas9.
Plant cell reports, 42(12): 2039-2042 PMID 37700173 DOI 10.1007/s00299-023-03050-5
Abstract
4382 available sgRNAs targeting 1060 tobacco genes were obtained, and 10,682 targeted mutants were created using high-throughput methods. Four optimization experiments were established to solve problems encountered during genetic transformation.