CRISPR/Cas9-Mediated Genome Editing in Zebrafish.
Methods in molecular biology (Clifton, N.J.), 2631: 371-380 PMID 36995678 DOI 10.1007/978-1-0716-2990-1_17
Abstract
The CRISPR/Cas9 system is a powerful tool for genome editing in zebrafish. This workflow takes advantage of the genetic tractability of zebrafish and will allow users to edit genomic sites and produce mutant lines using selective breeding. Established lines may then be employed by researchers for downstream genetic and phenotypic analyses.