Efficient Targeted Mutagenesis in Brassica Crops Using CRISPR/Cas Systems.
Methods in molecular biology (Clifton, N.J.), 2653: 253-271 PMID 36995631 DOI 10.1007/978-1-0716-3131-7_16
Аннотация
CRISPR/Cas has been established for targeted mutagenesis in many plant species since 2013, including Brassica napus and Brassica oleracea. Since that time, improvements have been made in terms of efficiency and choice of CRISPR systems. This protocol encompasses improved Cas9 efficiency and an alternative Cas12a system, allowing more challenging and diverse editing outcomes to be achieved.