CRISPR/Cas9.
Methods in molecular biology (Clifton, N.J.), 2637: 41-47 PMID 36773136 DOI 10.1007/978-1-0716-3016-7_3
Abstract
CRISPR/Cas9 is the genome-editing technology that is most widely used around the world. Its widespread adoption is largely due to its simplicity and ease of use. Here, we introduce the construction of vectors and genome editing of the target gene in cells using the CRISPR/Cas9 system.