Establishment of an efficient early flowering-assisted CRISPR/Cas9 gene-editing system in Arabidopsis.
Plant cell reports, 42(1): 211-214 PMID 36326850 DOI 10.1007/s00299-022-02944-0
Abstract
Ectopic expression of the florigen FT gene along with the Cas9 cassette promotes fast reproduction of the T1 transgenic plants, and the late-flowering phenotype serves as a convenient marker for transgene-free plants screening in T2 segregation population.