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High-throughput sequencing revealed low-efficacy genome editing using Cas9 RNPs electroporation and single-celled microinjection provided an alternative to deliver CRISPR reagents into Euglena gracilis.

Chen Z, Zhu J, Chen Z, Du M, Yao R, Fu W, Lei A, Wang J

Plant biotechnology journal, 20(11): 2048-2050 PMID 36036114 DOI 10.1111/pbi.13915