Histone gene editing probes functions of H3K27 modifications in mammals.
Nature genetics, 54(6): 746-747 PMID 35668297 DOI 10.1038/s41588-022-01096-x
Abstract
A new study employs CRISPR/Cas9-based base editing for simultaneous mutagenesis of all copies of histone H3 genes in mammals, highlighting the functional importance of H3K27me3 for Polycomb-mediated gene silencing and the dispensability for H3K27ac in transcriptional activation.