"X" marks the spot: Mining the gold in CasX for gene editing.
Molecular cell, 82(6): 1083-1085 PMID 35303481 DOI 10.1016/j.molcel.2022.02.024
Abstract
In this issue of Molecular Cell, Tsuchida et al. (2022) present a successful structure-guided effort in improving genome-editing efficiencies of CRISPR-CasX from Deltaproteobacteria (DpbCasX) and Planctomycetes (PlmCasX). Engineered variants that stabilize the active conformational state improved the catalytic efficiency by ∼10-20 fold in vitro and mean-editing efficiency by ∼2-3 fold in human cells.