[Advances of Cas9/sgRNA delivery system for gene editing].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 37(11): 3880-3889 PMID 34841792 DOI 10.13345/j.cjb.210040
Abstract
In the application of CRISPR genome editing, direct cellular delivery of non-replicable Cas9/sgRNA may reduce unwanted gene targeting and integrational mutagenesis, thus offering greater specificity and safety. Cas9/sgRNA delivery system holds great potential for treating genetic diseases. This review summarizes the advances of Cas9/sgRNA delivery systems and its therapeutic applications, providing new understandings and inspirations for vector design and future clinical applications.