Making the cut with PAMless CRISPR-Cas enzymes.
Trends in genetics : TIG, 37(12): 1053-1055 PMID 34563399 DOI 10.1016/j.tig.2021.09.002
Abstract
Genome editing technologies simplify our ability to rewrite genetic blueprints of life. However, CRISPR-Cas enzymes found in nature can only manipulate a fraction of the genome. To overcome this limitation, new Cas variants have been developed that unlock nearly the entire genome for editing.