Rice Haploid Inducer Development by Genome Editing.
Methods in molecular biology (Clifton, N.J.), 2238: 221-230 PMID 33471334 DOI 10.1007/978-1-0716-1068-8_14
Abstract
The current method to induce haploids in rice is anther culture, which is time-consuming and labor intensive and only works for some varieties. Here we describe a seed-based haploid induction system created by CRISPR/Cas9 technology. By editing OsMATL, we generate rice haploid inducer lines with a 2-5% haploid induction rate in different germplasms.