CRISPR/Cas9-Mediated Genome Editing of Trichoderma reesei.
Methods in molecular biology (Clifton, N.J.), 2234: 87-98 PMID 33165782 DOI 10.1007/978-1-0716-1048-0_8
Abstract
In this protocol, we describe the establishment of a CRISPR/Cas9 system in Trichoderma reesei by generating a specific, codon-optimized Cas9-expressing strain and by in vitro transcription of a gRNA. This system induces mutagenesis or introduces a gene in a targeted way based on PEG-mediated protoplast transformation. Up to three targets, multiplexed genome editing can be obtained in one transformation.