Gene Editing in the Ascidian Phallusia mammillata and Tail Nerve Cord Formation.
Methods in molecular biology (Clifton, N.J.), 2219: 217-230 PMID 33074543 DOI 10.1007/978-1-0716-0974-3_13
Abstract
Functional approaches for studying embryonic development have greatly advanced thanks to the CRISPR-Cas9 gene editing technique. Previously practiced in just a few organisms, these knockout techniques are now widely applied. Here we describe simple techniques for applying the CRISPR-Cas9 system to study the development of the nerve cord in the ascidian Phallusia mammillata.