Generation of Functional Genetic Study Models in Zebrafish Using CRISPR-Cas9.
Methods in molecular biology (Clifton, N.J.), 2174: 255-262 PMID 32813255 DOI 10.1007/978-1-0716-0759-6_16
Аннотация
CRISPR-Cas9 is a method for genome editing that can be used efficiently for in vivo applications; the basic implementation of this method is used to generate genome site-directed sequence eliminations. Here we describe a protocol for genome editing using CRISPR-Cas9 in zebrafish (Danio rerio) one-cell embryos.