Vaccinia Virus Genome Editing Using CRISPR.
Methods in molecular biology (Clifton, N.J.), 2023: 109-117 PMID 31240673 DOI 10.1007/978-1-4939-9593-6_6
Abstract
CRISPR/Cas9, an RNA-guided targeted genome editing system, can make precise, targeted modifications to the genome in living cells. Here we describe how this method can be used to efficiently edit the vaccinia virus genome enabling the insertion of transgene(s) specifically into a targeted site.