A guide for drug inducible genome editing with HIT systems.
Methods in enzymology, 621: 53-68 PMID 31128789 DOI 10.1016/bs.mie.2019.02.031
Abstract
Technologies toward precise control of biological events are desired for biomedical research and potential clinical applications. Our recently reported HIT systems based on CRISPR/Cas9 and TAL effectors can achieve temporal and dose dependent drug control for genome editing. Methods are presented for the application of these optimized HIT systems in human cells in this chapter.