Targeting Plant ssDNA Viruses with Engineered Miniature CRISPR-Cas14a.
Trends in biotechnology, 37(8): 800-804 PMID 31023561 DOI 10.1016/j.tibtech.2019.03.015
Аннотация
CRISPR-Cas14a is a highly compact protein with great potential to be used as a guided genome editing tool for single-stranded (ss) DNA cleavage. Recently isolated from noncultured archaea, its unrestricted and sequence-independent cleavage makes it an ideal tool for engineering resistance against economically important plant ssDNA viruses.