Genome Editing in Mice.
Methods in molecular biology (Clifton, N.J.), 1961: 249-254 PMID 30912050 DOI 10.1007/978-1-4939-9170-9_15
Аннотация
Programmable nucleases like CRISPR/Cas9 enable to edit the mouse genome directly in the zygote. Several methods have been successfully used for this. Here we describe injection into one of the pronuclei of the zygote and electroporation of zygotes. Alternative methods will be mentioned.