Gene Editing in 3D Cultured Nephron Progenitor Cell Lines.
Methods in molecular biology (Clifton, N.J.), 1926: 151-159 PMID 30742270 DOI 10.1007/978-1-4939-9021-4_13
Abstract
During kidney development, Six2+ nephron progenitor cells (NPCs) generate nephrons, the functional units of the kidney. Isolating and expanding NPCs in vitro have enabled wide applications in both basic and translational research. Here we describe the methods to derive NPC lines from mouse embryonic kidneys in a 3D culture format as well as gene editing in the NPC lines.