Analysis of Transcriptional Regulation in Bone Cells.
Methods in molecular biology (Clifton, N.J.), 1914: 145-167 PMID 30729464 DOI 10.1007/978-1-4939-8997-3_8
Аннотация
Transcription is a process by which the rate of RNA synthesis is regulated. Here we describe the techniques for carrying out promoter-reporter assays, electrophoretic mobility shift assays, chromosome conformation capture (3C) assays, chromatin immunoprecipitation assays, and CRISPR-Cas9 assay, five commonly used methods for studying and altering gene transcription.