Genome Editing in Citrus Tree with CRISPR/Cas9.
Methods in molecular biology (Clifton, N.J.), 1917: 235-241 PMID 30610640 DOI 10.1007/978-1-4939-8991-1_17
Abstract
CRISPR/Cas9 has been widely employed to edit genome in most of the organisms, including animal, plant, fungus, and microbe. Here we describe the modification of citrus gene CsLOB1 in transgenic citrus by Cas9/sgRNA, a two-component system derived from CRISPR-Cas9. Transgenic citrus plants can be created by Agrobacterium-mediated epicotyl transformation.