CRISPRing the Elephant in the Room.
Cell host & microbe, 24(6): 754-755 PMID 30543773 DOI 10.1016/j.chom.2018.11.015
Abstract
The importance of guanylyl-cyclases (GCs) in apicomplexa has remained elusive due to the large size of the genes. Two recent studies, including Brown and Sibley, 2018 in this issue of Cell Host & Microbe, make elegant use of genome editing with CRISPR-Cas9 to characterize roles of GCs in Toxoplasma and Plasmodium.