The Use of CRISPR-Cas9 Technology to Reveal Important Aspects of Human Airway Biology.
Methods in molecular biology (Clifton, N.J.), 1799: 371-380 PMID 29956165 DOI 10.1007/978-1-4939-7896-0_27
Abstract
The CRISPR-Cas9 technology is a powerful tool that enables site-specific genome modification (gene editing) and is increasingly used in research to generate gene knockout or knock-in in a variety of cells and organisms. This chapter provides a brief overview of this technology and describes a general methodology applicable to human airway biology research.