Genome Editing of Mouse by Cytoplasmic Injection.
Methods in molecular biology (Clifton, N.J.), 1630: 55-66 PMID 28643249 DOI 10.1007/978-1-4939-7128-2_5
Аннотация
CRISPR/Cas enables rapid production of genome-edited animals. The Cas9/gRNA component can be introduced to fertilized eggs in several ways. Here, we provide an instructional guide for the generation of knockout mice by cytoplasmic injection using in vitro transcribed Cas9 and gRNA.