Targeted Deletion of an Entire Chromosome Using CRISPR/Cas9.
Molecular therapy : the journal of the American Society of Gene Therapy, 25(8): 1736-1738 PMID 28633863 DOI 10.1016/j.ymthe.2017.05.021
Abstract
CRISPR/Cas9 genome editing can facilitate efficient deletion of genomic region, but it has not been used to delete an entire chromosome. Here, Adikusuma et al. show proof-of-concept for efficient CRISPR-mediated selective chromosome deletion by removing the centromere or shredding the chromosome arm in mouse embryonic stem cells and zygotes.